U.S. 301 South - Waldo to U.S. 441

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SR 24 branches 13.18 miles southwest from a grade separation along U.S. 301 at the CSX Railroad in Waldo to SR 20/26 (University Avenue) in Gainesville.
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SR 26 spans U.S. 301 at a grade separated intersection in Orange Heights.
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Passing north of Newmans Lake, SR 26 west leads to Gainesville Regional Airport (GNV) and the city of Gainesville.
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East from Orange Heights, SR 26 leads to SR 21 at Melrose near Lake Santa Fe and SR 100 at Putnam Hall.
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