State Road 45

State Road 45 forms a 300 mile long highway between Naples and High Springs along western reaches of the Florida peninsula. The route is vastly unsigned as the companion of U.S. 41 through Fort Myers, North Port, Sarasota, Bradenton, Brooksville and Williston. An exception occurs through Tampa, where SR 45 diverges from U.S. 41 as the counterpart to U.S. 41 Business by Port Tampa Bay and into the central business district.

A 3.17 mile long independent section of SR 45 in Tampa runs along Nebraska Avenue between U.S. 41 Business/SR 60 (Jackson Street / Kennedy Boulevard) and U.S. 41/92 (Hillsborough Avenue). Nebraska Avenue comprises an urban boulevard to the west of Ybor City, paralleling Interstate 275 north from the Downtown Interchange with I-4.